Review: imaging technologies for flow cytometry

Cell Interaction Analysis by Imaging Flow Cytometry

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Designing Hardware Accelerated Systems for Imaging Flow Cytometry

Lasers for flow cytometry.

Lasers are the principle light sources of flow cytometers. Most modern benchtop instruments feature air-cooled argon-ion laser emitting 10-25 mW of light at 488 nm. This unit covers the various types of lasers available and the qualities that make them suitable or unsuitable for use in flow cytometers. Also included is a discussion of future directions, particularly in the development of solid-...

Flow Cytometry

Flow cytometry is a technology that has impacted both basic cell biology and clinical medicine in a very significant manner. The essential principle of flow cytometry is that single particles suspended within a stream of liquid are interrogated individually in a very short time as they pass through a light source focused at a very small region. The optical signals generated are mostly spectral ...

Flow Cytometry

Because it has been difficult to identify and separate malignant cells in human lymphoid malignancies, we have developed a flow cytometry-based fluorescent in situ hybridization (FISH) technique using immunoglobulin (Ig) heavy chain variable region (V H) gene probes. After obtaining the specific V H gene sequence expressed by the multiple myeloma IM-9 cell line and the malignant cells in five m...